Part:BBa_K3726113

3CON5(H)_nNS5-down (PCC 11801)

This part is an homology 3’ connector harboring the down homology arm for the homologous recombination within the Neutral Site Nº 5 of PCC11801.

Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection: https://2019.igem.org/Team:Marburg/Part_Collection.

Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 3’ Connector parts can harbor the up / down homology arm required for double homologous recombination.

The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit.

Sequence & Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 575
  Illegal PstI site found at 839
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 575
  Illegal PstI site found at 839
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 505
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 575
  Illegal PstI site found at 839
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 575
  Illegal PstI site found at 839
  
• 1000
  COMPATIBLE WITH RFC[1000]

The genomic integration loci targeted by this part corresponds with a novel neutral site for Synechococcus PCC11801. This neutral site has been identified by the MADRID_UCM 2021 iGEM team as a part of their project. To know more about the discovery process visit the section Software of 4C_Fuels project. https://2021.igem.org/Team:MADRID_UCM/Software